One of the most important concepts and criteria of Good Laboratory Practices is accuracy, how well does a methodology stand in terms of quantifying when compared to an expected value?
There are various approaches in calibration and in the use of standards for comparisons. This webinar will cover validation of calibrations and the strengths, weaknesses, and appropriateness of the different types of standards.
Objectives of the Presentation
- How accuracy is determined
- What are the various types of standards and what are their strengths and weaknesses?
- The importance of traceability
- Calibration curves - what are the important criteria? Linearity versus nonlinearity, slope, intercept and what they mean
- Matrix effects and how to deal with them
- Using calibration data to monitor performance
Why Should You Attend:
Without accuracy, a result is not acceptable. There are many issues that affect accuracy and there are many approaches to deal with them. The importance of response, matrix effects, selectivity of identification, and other issues will be covered.
Areas Covered
- Comparison by using standards - the types of standards
- Traceability and documentation
- Calibration curves - checks for linearity, when non-linearity happens
- Spikes, surrogates, and standard addition
- Monitoring calibrations - slope and intercept
Instructor Profile:
Dr. John C. Fetzer has been doing liquid chromatographic method development for over 35 years. His PhD was in studies of various types of chromatography.
He has authored or co-authored over 50 papers on LC separations, has served on the advisory boards of the Journal of Chromatography, Analytical Chemistry, and Analytical and Bioanalytical Chemistry.
He supervised the Good Laboratory Practices accreditation of a large research chromatography laboratory and has taught numerous short courses on GLP and ISO 17025 compliance.